| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110667 |
| Chromosome: | chromosome 2 |
| Location: | 6864437 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119550 | NIP1 | NaCl-inducible protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCACCTAGGCCTTTGACCAGCACAATTG |
| Internal bar code: | GTCTTGCAAGCGCGGCACCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 852 |
| LEAP-Seq percent confirming: | 99.4322 |
| LEAP-Seq n confirming: | 2802 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCACCACAGGACCTGAA |
| Suggested primer 2: | CGACAAGATACCCACACACG |