| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110670 |
| Chromosome: | chromosome 3 |
| Location: | 6142709 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191900 | (1 of 1) K11840 - ubiquitin carboxyl-terminal hydrolase 9/24 [EC:3.4.19.12] (USP9_24) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGTCATGCACCACGACGTGAGCGGGC |
| Internal bar code: | CTAACGACTCCTCAAGGCACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 96.8421 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGCGCATAATCTCTG |
| Suggested primer 2: | GTTCATCAGCGCGTACAGAG |