Insertion junction: LMJ.RY0402.110703_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.110703
Chromosome:chromosome 14
Location:455557
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g610900 (1 of 1) IPR026205 - Progesterone-induced-blocking factor 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGCACCTAGGCGCCACGCCACTTGTCG
Internal bar code:ACGATGCCCACGAAGGTAATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:99.8092
LEAP-Seq n confirming:1046
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GACTAAACCAAACCAGGCCA
Suggested primer 2:GTGTGAGTGTGGGAGAGGGT