| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110705 |
| Chromosome: | chromosome 12 |
| Location: | 4227332 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g518900 | AKR9 | (1 of 4) 1.1.1.65 - Pyridoxine 4-dehydrogenase / Pyridoxine dehydrogenase; Aldo/keto reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCATCAAGCTGTTCACGTACGGCAGTGT |
| Internal bar code: | GACCCCTGGAATGCTTCATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 95.0428 |
| LEAP-Seq n confirming: | 5445 |
| LEAP-Seq n nonconfirming: | 284 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATCCACTCACCCGTGTA |
| Suggested primer 2: | AAACTGCTTGGTCACGTTCC |