| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110731 |
| Chromosome: | chromosome 14 |
| Location: | 1647695 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619050 | (1 of 1) PTHR31465:SF1 - PROTEIN RTA1-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCACTGTTTTTGGCTGAGCTGTGGTG |
| Internal bar code: | GTCGCCGGAGGTGCCACTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 98.4252 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCACAGGCTTTGACTTC |
| Suggested primer 2: | GACCACCCTAGAAGCAGCAG |