| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110746 |
| Chromosome: | chromosome 1 |
| Location: | 6446933 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045800 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGTTATGCCGGGCTTACATGTGTACAC |
| Internal bar code: | GTGTGTGCCGCGACGTCAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 99.1174 |
| LEAP-Seq n confirming: | 4380 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTGAACGAGTATCGTGA |
| Suggested primer 2: | AAGTGGGCGATGGTGTTAAG |