| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110755 |
| Chromosome: | chromosome 1 |
| Location: | 3999776 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025983 | protein of unknown function with DUF1399 domain; (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAATGAATAACCGGCCAAACGAAGTGA |
| Internal bar code: | ATAGGGGCATACCGTACACCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 99.384 |
| LEAP-Seq n confirming: | 484 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTACGACGGCAGTAACG |
| Suggested primer 2: | CATCATGCTGATGGCATTTC |