| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110759 |
| Chromosome: | chromosome 3 |
| Location: | 6048396 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191300 | LCI34,LCI35 | Low-CO2-inducible protein 35 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACTAGCCGTTGCCACACTCGTAAACTG |
| Internal bar code: | TTCCTGTGGTCAGCCGGATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 649 |
| LEAP-Seq percent confirming: | 99.6634 |
| LEAP-Seq n confirming: | 7698 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCAATTTACAAGAGCCA |
| Suggested primer 2: | CTTGCAAGCGATGTACCTGA |