Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.110787 |
Chromosome: | chromosome 11 |
Location: | 2678142 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g476700 | MPA9 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGAACCAGCGGAAGTGCGCCGACGTGT |
Internal bar code: | CTTCAACTGGCCGACACATGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 736 |
LEAP-Seq percent confirming: | 85.5525 |
LEAP-Seq n confirming: | 3097 |
LEAP-Seq n nonconfirming: | 523 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCCCACACCGTAACTCTCAT |
Suggested primer 2: | CTCTTCTCCGCCACATCTTC |