Insertion junction: LMJ.RY0402.110787_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.110787
Chromosome:chromosome 11
Location:2678142
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g476700 MPA9 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTTGAACCAGCGGAAGTGCGCCGACGTGT
Internal bar code:CTTCAACTGGCCGACACATGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:736
LEAP-Seq percent confirming:85.5525
LEAP-Seq n confirming:3097
LEAP-Seq n nonconfirming:523
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCACACCGTAACTCTCAT
Suggested primer 2:CTCTTCTCCGCCACATCTTC