Insertion junction: LMJ.RY0402.110798_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.110798
Chromosome:chromosome 2
Location:7310580
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g145350 (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCATTAACGTCACGTTTGCGCCGGGCA
Internal bar code:TGCCGTCGAGCCTATACAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:692
LEAP-Seq percent confirming:99.3066
LEAP-Seq n confirming:5442
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGATGATGAGACTGTGGG
Suggested primer 2:AAACACAGCAGCGACACATC