| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110812 |
| Chromosome: | chromosome 4 |
| Location: | 1575463 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCACCCACCGTGGCCACCGACGCCTGC |
| Internal bar code: | GGCCGCTCCCCTTTCGGCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 95.1002 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCACACCACACCGTCAG |
| Suggested primer 2: | GCAACGGGTACTTTAGCTGC |