| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110839 |
| Chromosome: | chromosome 2 |
| Location: | 4156422 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098150 | XYK1 | (1 of 1) 2.7.1.17 - Xylulokinase / Xylulose kinase; Xylulose kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACGGTCACGGCCGCCACATCAAAATG |
| Internal bar code: | GTTGACTCGAGGACCTTTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 98.5141 |
| LEAP-Seq n confirming: | 3116 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGTGGTGCAGTAGGCAT |
| Suggested primer 2: | CACCGTTCCCTGTAAGCAAT |