Insertion junction: LMJ.RY0402.110843_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110843
Chromosome:chromosome 12
Location:3018134
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g500850 (1 of 85) IPR009057 - Homeodomain-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGGTGGCACTGCCAGGCGCTGCCACAGG
Internal bar code:GGGCAAGGGTGGGGTTGTCTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:99.2298
LEAP-Seq n confirming:11595
LEAP-Seq n nonconfirming:90
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGTGCTGACTGAACTGAC
Suggested primer 2:GCTAACTCTGGCCACTGAGG