Insertion junction: LMJ.RY0402.110845_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.110845
Chromosome:chromosome 17
Location:829354
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g702150 TRX20,HCF164,CCS5 (1 of 1) PTHR10438//PTHR10438:SF272 - THIOREDOXIN // THIOREDOXIN-LIKE PROTEIN HCF164, CHLOROPLASTIC; Thioredoxin-like protein similar to Arabidopsis HCF164 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATTCGCACACATCAGTCACCCAAGACAA
Internal bar code:GGGTACGGTCCGGGGTCGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:98.5874
LEAP-Seq n confirming:4676
LEAP-Seq n nonconfirming:67
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGGGTGTATTGGGTGCTG
Suggested primer 2:AACCTTGGTGTTTCGTGAGG