| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110861 |
| Chromosome: | chromosome 10 |
| Location: | 4895796 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454650 | RPC5 | (1 of 1) K14721 - DNA-directed RNA polymerase III subunit RPC5 (RPC5, POLR3E); DNA-directed RNA polymerase III, 8 kD | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGTCTGCAGACTGGAGCGCATAAGTAGA |
| Internal bar code: | AACTCAACCTCGGACTTCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.9169 |
| LEAP-Seq n confirming: | 3609 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCCCAAGAAGCTGACTG |
| Suggested primer 2: | CTTGGCTGCCATGAACTTCT |