| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110864 |
| Chromosome: | chromosome 10 |
| Location: | 5361714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458183 | (1 of 3) 1.14.19.2 - Stearoyl-[acyl-carrier-protein] 9-desaturase / Stearyl-ACP desaturase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACGCGTGGGACTAGATGCCGTGCACCC |
| Internal bar code: | TCCAGCCGTCACGTCAACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.7037 |
| LEAP-Seq n confirming: | 4038 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTGACAGTCCAAGTAT |
| Suggested primer 2: | TTGCAGGATTAGGAAGGGTG |