Insertion junction: LMJ.RY0402.110877_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110877
Chromosome:chromosome 2
Location:2296948
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g090550 ARS9 Arylsulfatase; (1 of 1) PTHR10342//PTHR10342:SF228 - ARYLSULFATASE // EXTRACELLULAR SULFATASE SULF-1 HOMOLOG CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTCGGACCTGTGGGCTGCCATGGACGAG
Internal bar code:GCGTTGGCAAATTGAAGACGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:230
LEAP-Seq percent confirming:7.66083
LEAP-Seq n confirming:337
LEAP-Seq n nonconfirming:4062
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTCCTCTCCCTCTTCCTC
Suggested primer 2:CCGTGTCAAAATACACGCAC