Insertion junction: LMJ.RY0402.110920_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110920
Chromosome:chromosome 16
Location:6309861
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g674500 ABCA4 ABC transporter 4; (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGCGGCGACGGCGGCGGCGGCACCGCC
Internal bar code:CTTCAACGCCAACGCGGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:314
LEAP-Seq percent confirming:98.5675
LEAP-Seq n confirming:2890
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTTGGTTGATGGTTGTGG
Suggested primer 2:GAGGAGACAAGGACGTCAGC