Insertion junction: LMJ.RY0402.110920_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.110920
Chromosome:chromosome 17
Location:121136
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g697000 (1 of 2) PTHR23155:SF401 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGATGAACCCACTGACTCAACATCCTT
Internal bar code:TCCATTATGACGACTTCGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:208
LEAP-Seq percent confirming:99.5754
LEAP-Seq n confirming:938
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGGTAGTCTCGGAACGA
Suggested primer 2:GCTGTAGGTGTGGCTGTGAA