| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110928 |
| Chromosome: | chromosome 1 |
| Location: | 3124450 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019450 | SCE3,UBC9,UBE2I,CreSCE1c | (1 of 1) PTHR24067//PTHR24067:SF110//PTHR24067:SF51 - UBIQUITIN-CONJUGATING ENZYME E2 // SUBFAMILY NOT NAMED // SUMO-CONJUGATING ENZYME UBC9; SUMO-conjugating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGATCCTCATCGCCATGCAGGTGGGTT |
| Internal bar code: | GACTACAGGAGGTCGTGGCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 98.9603 |
| LEAP-Seq n confirming: | 4759 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGTTGCATTGCTGAAA |
| Suggested primer 2: | GTTTCCCCTTCACACAGCAT |