Insertion junction: LMJ.RY0402.110949_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110949
Chromosome:chromosome 2
Location:1996339
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g088350 SNR3 (1 of 6) PF09335 - SNARE associated Golgi protein (SNARE_assoc); SNARE associated Golgi protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTGGGCACCAGCCTTCGCAGCTCTGAA
Internal bar code:CCAGAGTGGGCCTACGTGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:658
LEAP-Seq percent confirming:98.1845
LEAP-Seq n confirming:2650
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCACAGCCTTTTTCTTC
Suggested primer 2:CAGTAGGGTGCTGTGGGAAT