| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110973 |
| Chromosome: | chromosome 14 |
| Location: | 2507821 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625251 | CSV12 | Chlamydomonas-specific family protein; (1 of 1) IPR006954 - Moulting cycle MLT-10-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCTGCAGCATGCCTGGCGCAATGGCGC |
| Internal bar code: | CGTTTCTTCCGAAAATGTCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 99.8192 |
| LEAP-Seq n confirming: | 552 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGGACAAGTGTGCTGACG |
| Suggested primer 2: | TCGGCAACATTGTACTCTGC |