| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110975 |
| Chromosome: | chromosome 17 |
| Location: | 1489418 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707000 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGACGCGGTCAAACCGCCAGCGCTTTG |
| Internal bar code: | TAAGTGGCTTAACTCACGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.0485 |
| LEAP-Seq n confirming: | 9473 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGCTGGCAGAGAGGTT |
| Suggested primer 2: | GTACTGCAGCCCAAGGAGAG |