| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111004 |
| Chromosome: | chromosome 3 |
| Location: | 988849 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148150 | (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGTGGTGCTGGTGGCAGGAGAGAGAGG |
| Internal bar code: | ATTGCAGCCATGGGCATAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 97.8627 |
| LEAP-Seq n confirming: | 12500 |
| LEAP-Seq n nonconfirming: | 273 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCATCCTAAACCAACG |
| Suggested primer 2: | GTCATGTACACCGGCACTTG |