| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111005 |
| Chromosome: | chromosome 7 |
| Location: | 1977323 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325755 | (1 of 1) PTHR33606:SF3 - PROTEIN YCII | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCGTGTGTGTGCACGTGACCACCGCC |
| Internal bar code: | GGAGTTGACCGGCCCGCCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 97.3649 |
| LEAP-Seq n confirming: | 2180 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGGGTGCTAGCCTTCTC |
| Suggested primer 2: | ATGAACGTGTATGGGGGAAA |