| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111019 |
| Chromosome: | chromosome 13 |
| Location: | 1279554 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570900 | MSRA1 | (1 of 2) PTHR10173:SF6 - PEPTIDE METHIONINE SULFOXIDE REDUCTASE; Peptide methionine-S-sulfoxide reductase, msrA-type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGGCGACGGGCTGCGGGCGTTGCAGCT |
| Internal bar code: | TCTGTGATGGTGTGCTACACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCACGAGTTGACGAGCA |
| Suggested primer 2: | GTTTGACCCTGCCATCATCT |