Insertion junction: LMJ.RY0402.111031_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111031
Chromosome:chromosome 17
Location:829671
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g702150 TRX20,HCF164,CCS5 (1 of 1) PTHR10438//PTHR10438:SF272 - THIOREDOXIN // THIOREDOXIN-LIKE PROTEIN HCF164, CHLOROPLASTIC; Thioredoxin-like protein similar to Arabidopsis HCF164 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGAAAACACTTGGAAGCAAAGCGGACC
Internal bar code:TGGTCAACTGCGTGGCAGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:540
LEAP-Seq percent confirming:90.0082
LEAP-Seq n confirming:9837
LEAP-Seq n nonconfirming:1092
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCATGCTGTACCGTATCT
Suggested primer 2:GAGGTTGTTACACCGGCAGT