| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111037 |
| Chromosome: | chromosome_13 |
| Location: | 5097906 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g607300 | MAPK5 | Mitogen-Activated Protein Kinase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCGACAACACCAAGTACACAGTCAGCGA |
| Internal bar code: | ACCGGCCGCCCTGTGGAGCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 98.8525 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACCTGTTGTGCTTGGGC |
| Suggested primer 2: | CTGCTGTAGTGCGTGTTGGT |