| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111037 |
| Chromosome: | chromosome 13 |
| Location: | 5097907 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607300 | FAP352,MAPK5 | (1 of 1) K08293 - mitogen-activated protein kinase [EC:2.7.11.24] (E2.7.11.24); Flagellar Associated Protein 352 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGGGCAGCACGATGATGCGGTTGCACAC |
| Internal bar code: | CTGTCAGCGCGTAGTGGTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 98.5294 |
| LEAP-Seq n confirming: | 268 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGTAGTGCGTGTTGGT |
| Suggested primer 2: | AATACCTGTTGTGCTTGGGC |