| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111040 |
| Chromosome: | chromosome 4 |
| Location: | 2688071 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGTAGCCCCTCACCAGCGGGGGCCCT |
| Internal bar code: | TGAGGTGACGTGAGCTAGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 98.5482 |
| LEAP-Seq n confirming: | 1154 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAAGCACCGTCGTCATCC |
| Suggested primer 2: | CGGGCATTCCTTCTTATCAA |