Insertion junction: LMJ.RY0402.111049_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.111049
Chromosome:chromosome 16
Location:3630074
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g685573 FAP214 Flagellar Associated Protein 214; (1 of 3) 3.1.1.4 - Phospholipase A(2) / Phospholipase A2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCGGACAAGCTCATGGGGTTAGGCATG
Internal bar code:GACTGTTAAAACACTGTGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:224
LEAP-Seq percent confirming:98.5801
LEAP-Seq n confirming:972
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGATGAGGGGTGTTACG
Suggested primer 2:CTTTCGCTAATTACGCAGCC