| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111050 |
| Chromosome: | chromosome 7 |
| Location: | 5167081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348350 | PWR8 | (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6); PWR motif protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTTGCGGCGCCGCCGATTCATCAACGCG |
| Internal bar code: | GGGACTGGGCTCCACAACGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 70.3626 |
| LEAP-Seq n confirming: | 1688 |
| LEAP-Seq n nonconfirming: | 711 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCAGCATACTGCTTTCA |
| Suggested primer 2: | TCATCTTGCTCCAACGTGTC |