| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111076 |
| Chromosome: | chromosome 10 |
| Location: | 2194674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433800 | CSE21 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTTGCGAGGGCGGCGCCAGTTGTCAAA |
| Internal bar code: | GCCGCAGACCGCCGCACTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 91.6575 |
| LEAP-Seq n confirming: | 1670 |
| LEAP-Seq n nonconfirming: | 152 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTCTAACACACACCGTA |
| Suggested primer 2: | ATCCGGATCTGGAAACTCCT |