Insertion junction: LMJ.RY0402.111092_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111092
Chromosome:chromosome_6
Location:6636292
Confidence (%):73
Locus disrupted Locus common name Defline Orientation Feature
Cre06.g293900 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CTGACGAGATGGGACGGTAGTTGAAGCTTG
Internal bar code:ATGGACTCGGTCTGATGGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:199
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:170
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGCCCTCTGAGTACTTG
Suggested primer 2:TACCGTACTCGACCCCACTC