| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111097 |
| Chromosome: | chromosome 8 |
| Location: | 1670376 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365692 | SIR4,SIR2 | Ferredoxin-sulfite reductase; (1 of 2) 1.8.7.1 - Assimilatory sulfite reductase (ferredoxin) / Sulfite reductase (ferredoxin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGTTCGCGGCAAGGTGGTGTGCGTTA |
| Internal bar code: | GGGAACGGCGAAGGTATGGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 710 |
| LEAP-Seq percent confirming: | 77.1131 |
| LEAP-Seq n confirming: | 3622 |
| LEAP-Seq n nonconfirming: | 1075 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCTGGGAGTAACAGCAG |
| Suggested primer 2: | GCCTACTGGTATAGCTGCGG |