Insertion junction: LMJ.RY0402.111103_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111103
Chromosome:chromosome 16
Location:3862691
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g689950 (1 of 1) PF00168//PF08372 - C2 domain (C2) // Plant phosphoribosyltransferase C-terminal (PRT_C) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAGTGAGTCTGCGGAGGCCGGGGCTGG
Internal bar code:AAGTAAGGTCGATCGCGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:408
LEAP-Seq percent confirming:93.3011
LEAP-Seq n confirming:3092
LEAP-Seq n nonconfirming:222
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTTGCACCGTATGCCTTT
Suggested primer 2:GGCTATGCCATCGCTAGAAG