| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.111131 |
| Chromosome: | chromosome_10 |
| Location: | 1321598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g427600 | KAT1 | Katanin catalytic subunit, 60 kDa | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGACCCGAGCGTGCCAGAGCCTGCCATGT |
| Internal bar code: | TTAGCATAGACCAAGTTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 98.234 |
| LEAP-Seq n confirming: | 445 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCTTTAGAGCACAGGGG |
| Suggested primer 2: | AGCTTGTTGTCATCCTGGCT |