| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111134 |
| Chromosome: | chromosome 3 |
| Location: | 1487645 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151650 | SMM7 | (1 of 14) PF10294 - Lysine methyltransferase (Methyltransf_16); S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCGCCACCGCCCGAGCACGTACGGCTT |
| Internal bar code: | TCTAAAATATGGAATTTTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCCCTAGCACAACAACT |
| Suggested primer 2: | TTACAGAAAGGGGTCGTTGG |