| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111146 |
| Chromosome: | chromosome 4 |
| Location: | 1273282 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215001 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGAGGTGGGTGGTGTGGTGTGGTGTGG |
| Internal bar code: | CGTACGGCTGGCGAAGGTGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 1.49128 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 9380 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGCTGCACATTTCTCCA |
| Suggested primer 2: | GGAAGTGGGTGTGGTAGGTG |