| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111157 |
| Chromosome: | chromosome 6 |
| Location: | 6908081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295826 | (1 of 1) IPR002048//IPR007065 - EF-hand domain // HPP | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATTGGTTTAGGGCAGCATTAGTAATGC |
| Internal bar code: | TTGGGTCGGACCATCACAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCCCGGTTAGGATCAAC |
| Suggested primer 2: | CCTGCGGCACTAGTTTCTTC |