Insertion junction: LMJ.RY0402.111161_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.111161
Chromosome:chromosome 12
Location:9009367
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g543700 TNP32 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCGAGGACACGCGGCGCAGGCCGCCGGT
Internal bar code:GCGAGCTTTTCAACCCAATGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:109
LEAP-Seq percent confirming:91.5385
LEAP-Seq n confirming:119
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTCAACAATGCGTATCC
Suggested primer 2:CAGCGAGTGGTTCTTAAGGC