| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111173 |
| Chromosome: | chromosome 1 |
| Location: | 626636 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003508 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTAGAGAACGAGGAAAGAAGTGCGACG |
| Internal bar code: | CGTCTGACTCAGCACCGCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1065 |
| LEAP-Seq percent confirming: | 98.6877 |
| LEAP-Seq n confirming: | 376 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAACACGTCCTGGAAGACC |
| Suggested primer 2: | TACAGGGCATCGTCGTGTTA |