| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111176 |
| Chromosome: | chromosome 6 |
| Location: | 3178394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATTGTTACTGCTTCATTGTCAGTTGCC |
| Internal bar code: | CGCGAAGGATGCAGGATCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 99.2163 |
| LEAP-Seq n confirming: | 3418 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCAATAACCCCACAACG |
| Suggested primer 2: | TTGGTACCTTCTTGGCCATC |