| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111190 |
| Chromosome: | chromosome 12 |
| Location: | 9333290 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540650 | (1 of 2) PTHR19241:SF62 - PROTEIN SCARLET | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACACATGCGCCCCACAATCCACCCA |
| Internal bar code: | GTCGGGTAACACGCTCGGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 56.9633 |
| LEAP-Seq n confirming: | 2221 |
| LEAP-Seq n nonconfirming: | 1678 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCCGGGTTGAAGTAGTA |
| Suggested primer 2: | GGTACTCCATCCCGGAACTT |