Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.111196 |
Chromosome: | chromosome_10 |
Location: | 2921382 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g440250 | PGM19 | sense | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGAACATCGTCAGCACCCAGTCGCTGCTC |
Internal bar code: | GGGGGAAAGATCAAGACGGTGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 417 |
LEAP-Seq percent confirming: | 98.9169 |
LEAP-Seq n confirming: | 3105 |
LEAP-Seq n nonconfirming: | 34 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACGGCGAGTTGAAAGAAGA |
Suggested primer 2: | GTAACCCTCCCTGCACGTTA |