| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111242 |
| Chromosome: | chromosome 16 |
| Location: | 4063079 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669900 | FAP399 | Flagellar Associated Protein 399 with Low in Lung Cancer domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGTATCGAAGCCTTTGACGCCACCGCA |
| Internal bar code: | AGGAATTCGCTGCGTAGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 99.7998 |
| LEAP-Seq n confirming: | 997 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATATAGGCAATCGGGGAT |
| Suggested primer 2: | CAAGGTGCGAGTATGCGTTA |