| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.111244 |
| Chromosome: | chromosome 8 |
| Location: | 2305204 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370350 | (1 of 6) PF09409 - PUB domain (PUB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGAAGTCCAGGGCCTCATATGTTCGG |
| Internal bar code: | GTAAATGGTTCAGCGTTTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1062 |
| LEAP-Seq percent confirming: | 99.7132 |
| LEAP-Seq n confirming: | 10083 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTTGAGCACTGACGGAC |
| Suggested primer 2: | ACTTGTTGGTACCTGCGGAC |