| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.111260 |
| Chromosome: | chromosome 1 |
| Location: | 508119 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g002861 | SRR24 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAGCAGTAGATGGGGCAGAAGCAAGACT |
| Internal bar code: | CTCTGAAGTTGGCGGAACGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 94.9068 |
| LEAP-Seq n confirming: | 7789 |
| LEAP-Seq n nonconfirming: | 418 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTACTCGCTGGGAACC |
| Suggested primer 2: | TGCTACTGCAGACCCTGATG |