Insertion junction: LMJ.RY0402.111281_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.111281
Chromosome:chromosome 16
Location:3810737
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g689550 PTK8 Putative tyrosine kinase; (1 of 10) PTHR23257//PTHR23257:SF520 - SERINE-THREONINE PROTEIN KINASE // IP11267P 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCCACCGTGTGAAAACCACCATTTGCCTT
Internal bar code:CCGATCTGGATTTGTCTGTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:934
LEAP-Seq percent confirming:97.5207
LEAP-Seq n confirming:118
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCATTTTAGGATGTCGG
Suggested primer 2:GACTTCACTTGCCTGCATCA