| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.111284 |
| Chromosome: | chromosome 11 |
| Location: | 1404916 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467723 | KAS1 | (1 of 1) PTHR11712//PTHR11712:SF226 - POLYKETIDE SYNTHASE-RELATED // 3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE I, CHLOROPLASTIC; 3-ketoacyl-ACP-synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCTCCCGCAAGACACTCTTCATTGCAAC |
| Internal bar code: | GATCCGCGTCAGAGGCGCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.0527 |
| LEAP-Seq n confirming: | 5228 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCTGAGGTTCCTGTCG |
| Suggested primer 2: | CTCAAATTCGAGTCCTTCGC |